GSM7720564: RD shSKP2, MYOD, ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MYOD1
Cell type
Cell type Class
Muscle
Cell type
RD
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma
Attributes by original data submitter
Sample
source_name
RD
cell line
RD
cell type
Fusion-Negative Rhabdomyosarcoma
genotype
SKP2 knockdown
chip-antibody
MYOD (CST, #13812)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7720564
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixes with 1% formaldehyde for 10 min. After quenching, cells were digested to achieve chromatin fragmented to a range of 150–900 bp using Micrococcal Nuclease. Chromatin samples were immunoprecipitated overnight at 4 °C. DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit, following manifacturer's instruction.